Laboratory Methodology in BiochemistryCRC Press, 27/12/1989 - 280 من الصفحات Provides information on methodologies and techniques concerning the biochemical laboratory, as well as improvements or advancements made on existing methodologies. Original methodologies for the purification of biological macromolecules and methodologies for metabolic pathways and enzyme kinetics are covered. The application of biochemical and biophysical methodologies for the structural and dynamic characterization of biological macromolecules is considered. The elaboration of automated systems for biochemical research and computer programs for the management and processing of experimental data are both reviewed. Development of instruments and equipment for biochemical research is also presented. |
المحتوى
19501970 | 2 |
References | 8 |
Chapter | 9 |
Amino Acid Analysis Using the Dimethylaminoazobenzene Sulfonyl Chloride DABSCI | 35 |
Chapter 5 | 63 |
Chapter 6 | 83 |
Chapter 7 | 109 |
Chapter 8 | 129 |
Chapter 9 | 151 |
ASPN Endoproteinase A New Specific Tool in Protein Sequencing | 173 |
Chapter 12 | 207 |
Chapter 14 | 231 |
| 257 | |
طبعات أخرى - عرض جميع المقتطفات
عبارات ومصطلحات مألوفة
50 pmol a-tubulin Acad acetate acetonitrile algorithm alignment amino acid analysis amino acid composition amino acid sequence ammonium bicarbonate Anal analyzed Biochem Biol Biophys blotting buffer Chem chromatogram cleavage cleaved concentration cycle DABITC DABTH amino acids derivatives derivatization detection determination Edman degradation electroblotted elution enzymatic enzyme extraction FAB mass spectrometry FEBS Lett flow rate fractions fragments gas-phase gradient HPLC hydrolysis hydrophobic identification incubated injected ion signals isolated liquid chromatography mass spectrometry mass spectrum membrane microsequencing microtubules mixture ml/min modification molecular weight N-terminal Natl obtained peaks phosphorylation picomole PITC polypeptide posttranslational modifications precolumn derivatization prediction primary structure Proc protein kinase protein sequencing PTH-amino acids purification quantitative reagent residues reverse-phase HPLC sample secondary structure sensitivity separation sequence analysis shown in Figure sodium solvent substrate subunit technique transferrin trifluoroacetic acid trypsin tryptic digest tryptic peptides tube tubulin urea yield
مقاطع مشهورة
الصفحة 255 - Roepstorff, P. and Fohlman, J. (1984) Proposal for a common nomenclature for sequence ions in mass spectra of peptides.